About the project
The main objective of this project is to develop vesicle systems to produce and excrete ATP and precursors for lipids, and to achieve membrane expansion by lipid synthesis. We will exploit the properties of condensates to enhance the efficiency of reaction networks for fuel supply and lipid building blocks inside vesicles.
The project will include secondments at RU, NL (Spruijt group, spruijtlab.com; Compartmentalization of reactions), the MPI in Potsdam, FRG (Dimova group, www.dimova.de; Biophysical analysis of membranes) and MPI in Martinsried, FRG (Schwille group, www.biochem.mpg.de/schwille, Microfluidics technology).
Further information on the interests of the Poolman group can be found on www.membraneenzymology.com.
Selected References
- Heinen L, van den Noort M, King MS, Kunji ERS & Poolman B (2024) Synthetic syntrophy: A platform technology for adenine nucleotide cross-feeding between metabolically active vesicles. Nature Nanotech, in press. doi.org/10.21203/rs.3.rs-3990950/v1
- Patino-Ruiz M, Anshari ZR, Gaastra B, Slotboom DJ & Poolman B (2024), Chemiosmotic nutrient transport in synthetic cells powered by electrogenic antiport coupled to decarboxylation, Nature Comm 15: 7976. https://doi.org/10.1038/s41467-024-52085-z
- Śmigiel WM, Mantovanelli L, Linnik DS, Punter CM, Silberberg J, Xiang L, Xu K & Poolman B (2022) Synthetic syntrophy for adenine nucleotide cross-feeding between metabolically active nanoreactors. Science Adv 8:eabo5387. DOI: 10.1126/sciadv.abo5387
Doctoral Candidate: Aditi Kakkad
Aditi completed her B.Sc. at the University of Hyderabad and her Master’s degree at the Weizmann Institute of Science in Israel.
At Weizmann, she investigated protein folding within the GroEL/ES chaperonin system, using kinetic and thermodynamic approaches to elucidate folding pathways within the confined chaperonin cavity.
Hosted by:
Bert Poolman
University of Groningen, The Netherlands
Department of Biochemistry
www.membraneenzymology.com
en.wikipedia.org/wiki/Bert_Poolman
